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. 2018 Jul 19;8:10907. doi: 10.1038/s41598-018-29367-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flowchart of the ECLIPSE algorithm. In light blue are highlighted the pre-processing steps (0a: pre-processing on the raw MFC data, 0b: pre-processing on the raw MFC data, after removal of normal cells); in red the probability densities distribution estimations (2A: estimate of control PDF, 2B: estimate of responder PDF, from the projection of responder-group cells); in grey the results of the corresponding previous steps.