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. 2018 May 24;8:41. doi: 10.1038/s41387-018-0035-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Female C57BL/6J mice were divided into four groups and fed with the normal fat (NF) no betaine (BC) diet, NF betaine-supplemented (BS) diet, HF-BC diet, or HF-BS diet for 4 (cohort 1) or 6 (cohort 2) weeks before timed-mating and throughout gestation. Male mice follow the NF-BC diet until timed-mating. The intraperitoneal glucose tolerance test (GTT) was conducted on embryonic day (E) 11.5 and 15.5. Dissection was conducted on E12.5 (cohort 1) or E17.5 (cohort 2). n is the number of dams in each group from which data and samples were collected