Fig. 7. Skin inflammation in mtDNA-depleter mice.

a Immunocytochemical and histochemical analyses of skin sections show the presence of increased number of inflammatory cells including mast cells (Giemsa stain-positive cells), granulocytes (MPO-positive cells), macrophages and histiocytes (CD163-positive cells), and B lymphocytes (Pax-5-positive cells) in the dermis, as well as in perifollicular and periepidermal location of mtDNA-depleter mice. The skin sections of wild-type mice are predominantly negative for MPO, CD163, and Pax-5 staining. Arrows indicate the presence of inflammatory cells in the skin sections. b Quantitative analysis of Giemsa-positive mast cells in the skin sections of wild-type control and mtDNA-depleter mice (mean ± s.e.m; *P<0.05, Student’s t test). c RT-PCR analysis of inflammatory genes in the skin RNA samples of wild-type control (WT; n = 3) and mtDNA-depleter mice (Depleter; n = 3) after 2 months of continuous dox induction. d RT-PCR analysis of genes in the skin RNA samples of wild-type control (n = 3) and mtDNA-depleter mice (n = 3) after 2 months of continuous dox induction. Depleter = mtDNA-depleter mice