Table 2.

CCD with observed and predicted values of CO activity.

Yeast Extract	Dextrose	Starch	Ammonium carbonate	Enzyme activity U/ml
				Observed	Predicted	Residual
0.4	0.4	0.8	0.02	1.694	1.698545	−0.004545
0.4	0.4	0.8	0.02	1.7	1.698545	0.001455
0.6	0.6	0.4	0.01	2.168	2.164430	0.003570
0	0.4	0.8	0.02	0.66	0.788276	−0.128276
0.2	0.2	0.4	0.01	0.76	0.749693	0.010307
0.4	0.4	1.6	0.02	1.96	2.097274	−0.137274
0.4	0.4	0.8	0	1.25	1.326244	−0.076244
0.8	0.4	0.8	0.02	2.788	2.823809	−0.035809
0.2	0.2	1.2	0.01	1.534	1.394519	0.139481
0.4	0.4	0.8	0.02	1.7	1.698545	0.001455
0.4	0	0.8	0.02	1.33	1.402118	−0.072118
0.4	0.4	0.8	0.04	1.8	1.854525	−0.054525
0.6	0.2	1.2	0.03	2.427	2.344019	0.082981
0.6	0.2	0.4	0.03	2.023	2.019173	0.003827
0.4	0.8	0.8	0.02	1.926	1.977144	−0.051144
0.4	0.4	0.8	0.02	1.69	1.698545	−0.008545
0.4	0.4	0.8	0.02	1.7	1.698545	0.001455
0.4	0.4	0	0.02	1.35	1.283244	0.066756
0.4	0.4	0.8	0.02	1.71	1.698545	0.011455
0.4	0.4	0.8	0.02	1.7	1.698545	0.001455
0.4	0.4	0.8	0.02	1.69	1.698545	−0.008545
0.2	0.6	1.2	0.03	1.855	1.743433	0.111567
0.2	0.6	0.4	0.03	1.2	1.193526	0.006474
0.6	0.6	1.2	0.01	1.939	1.832878	0.106122
0.4	0.4	0.8	0.02	1.71	1.698545	0.011455
0.4	0.4	0.8	0.02	1.7	1.698545	0.001455
0.4	0.4	0.8	0.02	1.69	1.698545	−0.008545

Note: The concentration units of yeast extract, dextrose, starch and ammonium carbonate were in g/100 ml.