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. 2018 Jul 19;8:10960. doi: 10.1038/s41598-018-27934-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effects of Au-NPs on cardiac histological changes pronounced by HE stain in normal and ISO-induced hyperthyroid heart disease groups. HE (magnification, x20) staining was performed to determine general histological alterations in control and ISO-induced hyperthyroid heart disease groups. Red part represented muscle cytoplasm while blue part demonstrated nucleus in HE stains. The histological heart alterations induced by Au-NPs were size-dependent with 40 nm AuNPs demonstrated prominent congested dilated blood vessels in control groups, while for HHD groups, the pathological changes were decreased with few lymphocytic infiltrate and less disturbed muscle fascicles when combined with 5 nm and 100 nm application (n = 6).