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. 2018 Jul 19;8:10960. doi: 10.1038/s41598-018-27934-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effects of Au-NPs on cardiac left ventricular collagen volume pronounced by Masson stain in normal and ISO-induced hyperthyroid heart disease groups. Masson (magnification, x20) staining was performed to determine cardiac fibrosis in control and ISO-induced hyperthyroid heart disease groups. Red part represented muscle tissue while blue part demonstrated fibrosis in Masson stain. The fibrosis volume induced by Au-NPs was size-dependent with 40 nm AuNPs induced more effects in control groups, while for ISO-induced hyperthyroid heart disease groups, more pronounced fibrosis volume was caused but decreased when combined with 5 nm and 100 nm AuNPs application (n = 6).