Fig. 3.

FFN270 labels NE neurons in the locus coeruleus. a Atlas image highlighting in red the location of the locus coeruleus in the mouse brain (Bregma: −5.5 mm, Allen Institute)⁵¹. b–d FFN270 (magenta, b) effectively colocalized with the noradrenergic label TH-GFP (green, c), resulting in a 72% colocalization of noradrenergic cell bodies (labeled by red arrows, 62/86 cells, 6 animals). Cells that did not colocalize are highlighted with a blue arrow, and blood vessels are highlighted with a yellow arrow. e–g When repeated with FFN102 (red, e), no colocalization was observed with TH-GFP (f). Images obtained by 2-photon microscopy in acute mouse brain slice (for imaging parameters, see Methods). Scale bar: 10 µm