Figure 4.

Apoptotic signaling in response to DNA-PK inhibition and ATR inhibition in combination with radiation. (A) Western blot analysis was used to assess levels of H2Ax phosphorylation on Ser139, and apoptosis was identified by cleaved caspase 3 and cleaved PARP. Cell lysates were harvested at 48 h after radiation with AZD6738 and KU-0060648 addition 1 h before radiation. (B) Sub-G1 cells were identified at 24 and 48 h by propidium iodide staining and FACS in response to AZD6738, KU-0060648, and 4 Gy radiation combinations. Data represent a minimum of three independent experiments ± SEM. Statistical analysis performed between indicated conditions by unpaired t-test *P < 0.05.