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. 2018 Jul 13;9:930. doi: 10.3389/fpls.2018.00930

FIGURE 2.

FIGURE 2

(A) AtWRKY50 induced expression is independent of NPR1 and TGAs. Protoplasts from wild type (WT), tga2-1 tga5-1 tga6-1 triple mutant (tga256) and npr1-1 mutant (npr1) plants were co-transfected with PR1::GUS reporter construct alone (minus sign) or together with expression plasmids containing 35S::AtWRKY50 (W50), as indicated. After incubation GUS activity was measured spectrophotometrically. The bars represent the percentage of GUS activity from triplicate experiments relative to that of the protoplasts co-transfected with the PR1::GUS construct and empty vector control. Error bars represent the SEM. Asterisk (∗) shows the statistical differences among samples (p < 0.05). (B) Synergistic effect of AtWRKY50 and TGA2 or TGA5 on PR1 expression. Arabidopsis protoplasts were co-transfected with PR1::GUS reporter construct alone (minus sign) or together with expression plasmids containing 35S promoter-controlled genes encoding AtWRKY50, TGA2, TGA3, TGA5, or combinations, as indicated. After incubation GUS activity was measured spectrophotometrically. Expression levels (%) are given relative to the expression the level without expression plasmid. The bars represent the percentage of GUS activity from triplicate experiments relative to that of the protoplasts co-transfected with the corresponding PR1::GUS construct and empty vector control. Error bars represent the SEM. Asterisk (∗) shows the statistical differences among samples (p < 0.05).