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Parameters of the mono-exponential function used to fit the fluorescence recovery curve on spread and roundish cells: t1 is the characteristic time of the GFP protein translocation from cytosol to the nucleus; A1 corresponds to the fraction of protein involved in the exchange; and y0 is the fluorescence background.
| Sample | y0 (a.u) | A1 (a.u) | t1 (s) | σy0(a.u) | σA1 | σt1 (s) |
|---|---|---|---|---|---|---|
| Roundish cells | 0.6 | −0.6 | 26 | 0.004 | 0.008 | 2.6 |
| Spread cells | 0.4 | −0.3 | 56 | 0.003 | 0.007 | 2.5 |
