Fig. 2.

A ΔpgpB mutant shows increased susceptibility to cefsulodin. (A) The growth inhibition zone around discs loaded with 30 µg of cefsulodin was significantly increased in the mutant strain lacking PgpB compared to wt strain (BW25113) or strains lacking BacA, YbgG or PBP1A (ΔmrcA). As expected, the mutant lacking PBP1B (ΔmrcB) was hyper-susceptible to cefsulodin. The data are mean ± SD of three independent experiments. (B) Spot plate assay showing the increased susceptibility of the ΔpgpB mutant on plates containing 32 µg/mL cefsulodin.