Fig. 7.

C55-PP inhibits the GTase activity of PBP1B. (A) Continuous fluorescence assay for GTase activity of PBP1B in the presence of C55-PP synthesized by incubating UppS and its substrates C5-PP and C15-PP (+UppS/S). Samples contained PgoB, PgpB(D211E) (PgpB*) or no phosphatase; control samples contained only UppS or substrates, or the known GTase inhibitor moenomycin (Moe). (B) GTase activity assay of PBP1B in the presence or absence of C55-P. (C) The GTase rates obtained from the assays in (A) and (B) and control reactions in Fig. S4B–D were normalised relative to the activity of PBP1B in the absence of PgpB, UppS and its substrates. ^*p < 0.05; ^**p < 0.005. The values are mean ± SD of at least 3 independent experiments.