Fig. 1.

(A) Structure of moenomycin A (MoeA). (B) Overall view of the re-refined structure of SaMGT E100Q mutant with bound MoeA (PDB ID code 6FTB). The jaw subdomain is colored yellow and head subdomain is colored orange. ${\text{MoeA}}_{\mathit{DON}}$ (green) is shown in stick representation and ${\text{MoeA}}_{\mathit{JAW}}$ (pink) is shown in ball-and-stick representation. Polder electron density map (Liebschner et al., 2017) for ${\text{MoeA}}_{\mathit{DON}}$ (grey mesh) and ${\text{MoeA}}_{\mathit{JAW}}$ (blue mesh) is contoured at 2$\sigma$. Unmodelled parts of ${\text{MoeA}}_{\mathit{JAW}}$ (due to no visible electron density) is shown in gray colored line representation. (C) SaMGT with bound lipid II analog (cyan colored shown in stick representation) in the acceptor site (PDB ID code 3VMT) and the PGT domain of Aquifex aeolicus PBP1A (PDB ID code 2OQO) with bound CHAPS (black colored shown in stick representation) are superimposed on 6FTB. Only 6FTB (gray colored cartoon) is shown for clarity. ${\text{MoeA}}_{\mathit{DON}}$ and ${\text{MoeA}}_{\mathit{JAW}}$ are shown as in B. PGT motifs I to V (colored red) and flap (colored yellow) is shown. (For interpretation of the references to colour in this figure caption, the reader is referred to the web version of this article.)