Fig. 2.

Energetics of cooperative sequential binding of MoeA to SaMGT. ITC heat response (top) and binding isotherm (bottom) of the calorimetric titration of MoeA into SaMGT. A cartoon representation of SaMGT (head subdomain colored orange and jaw subdomain colored yellow) and MoeA (pink) is shown in the bottom chart. The solid line in the bottom chart represents the fit to a sequential binding site model. The dissociation constant $({\text{K}}_d)$ for the ${\text{MoeA}}_{\mathit{JAW}}$ and ${\text{MoeA}}_{\mathit{DON}}$ binding steps are 2.8 μM and 0.54 μM, respectively. (For interpretation of the references to colour in this figure caption, the reader is referred to the web version of this article.)