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. Author manuscript; available in PMC: 2018 Jul 20.
Published in final edited form as: Metab Eng. 2016 May 10;37:72–78. doi: 10.1016/j.ymben.2016.05.005

Figure 1.

Figure 1.

Selection of isotopic tracers for quantifying the TKTL1 flux. The fate of glucose carbon atoms is tracked for four flux scenarios: (A) glycolysis; (B) oxPPP; (C) maximum TKTL1 flux fueled by oxPPP; and (D) maximum TKTL1 flux fueled by noxPPP. (E) The expected ratios of mass isotopomers (M1+2*M2)/M3 for 3PG and PEP for all four flux scenarios and three tracer combinations: [1-13C]glucose+[4,5,6-13C]glucose, [2-13C]glucose+[4,5,6-13C]glucose, and [3-13C]glucose+[4,5,6-13C]glucose. The (M1+2*M2)/M3 ratio for the first tracer combination is related to oxPPP flux, and the (M1+2*M2)/M3 ratio for the last two tracer combinations is related to TKTL1 flux.