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. Author manuscript; available in PMC: 2018 Jul 20.
Published in final edited form as: Cell Rep. 2018 Jan 9;22(2):512–522. doi: 10.1016/j.celrep.2017.12.050

Figure 1. Carbon for 2-HG Synthesis Can Be Derived from Glucose or Glutamine.

Figure 1

(A) Relative level of 2-HG in wild-type HCT116 cells grown with 25 mM glucose and no glutamine (other than that from FBS), or 4.5 mM glutamine and no glucose (other than that from FBS). Irrespective of condition, the cells synthesized 2-HG.

(B) Isotopologue distribution pattern of 2-HG after HCT116 R132H/+ cells were labeled with U-13C glutamine, with no glucose (other than that from FBS) present in the culture media.

(C) Isotopologue distribution pattern of 2-HG after HCT116 R132H/+ cells were labeled with U-13C glucose, with no glutamine (other than that from FBS) present in the culture media.

(D) Isotopologue distribution pattern of 2-HG after HCT116 R132H/+ cells were labeled with U-13C glucose in the presence of 4.5 mM glutamine. Data shown are mean values ± SD (n = 3).