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. 2018 Jul 4;145(13):dev164855. doi: 10.1242/dev.164855

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© 2018. Published by The Company of Biologists Ltd

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Fig. 1. — Sox30 is highly enriched in mouse testes. (A-C) Western blot analysis of Sox30 protein in lysates from adult mouse tissues (A), from mouse testis tissue collected at different time points during postnatal development (B) and from isolated spermatogenic cell populations, including Sertoli cells (SE), spermatogonia (SG), pachytene spermatocytes (PS), round spermatids (RS) and elongating spermatids (ES). β-actin serves as an internal loading control. (D,E) Immunostaining of testis sections from 8-week-old wild-type (D) and Sox30^−/− mutant (E) mice for Sox30 (green) and PNA (red). PNA is an acrosome marker. DNA was counterstained with DAPI. Lower panels show magnifications of the boxed area in the upper panels. Dip, diplotene; ES, elongating spermatids; Pac, pachytene; pre-lep, pre-leptotene; RS, round spermatids; Zyg, zygotene. Scale bars: 50 μm. (F) Graphic representation of Sox30 expression (green bar) during spermatogenesis.