Fig. 3.
Hoxb8 microglia progenitors are selectively expanded during AGM and fetal liver hematopoiesis. (A) FACS profiles of CD41+ cells gated for c-Kit and tdTomato from the yolk sac of E8.5 and E9.5 Cx3cr1GFP/+; Hoxb8IRES-Cre/+; ROSA26CAG-LSL-tdTomato/+ embryos. (B) FACS profile of CD41+ cells gated for c-Kit and tdTomato isolated from the AGM of E10.0 and E11.5 embryos. (C) FACS profiles of CD45+ cells gated for c-Kit and tdTomato from fetal livers of E14.5 and E18.5 Cx3cr1GFP/+; Hoxb8IRES-Cre/+; ROSA26CAG-LSL-tdTomato/+ embryos. (D) Graph showing the percentage of Hoxb8 hematopoietic progenitor cells (CD41+ c-Kithigh tdTomato+) through embryonic development in different tissues. n=2-4 replicates or pooled replicates per time point; data are mean±s.e.m. See also Fig. S1. (E) Graph showing the percentage of Hoxb8 f-HSCs (CD45+ Sca-1+ c-Kithigh tdTomato+) through development in different tissue. n=2-6 replicates or pooled replicates per time point; data are mean±s.e.m. (F) Graph depicting the percentage of Hoxb8 HSCs (Lin− Sca-1+ c-Kithigh tdTomato+) in adult bone marrow. n=5 replicates; data are mean±s.e.m. See also Figs S1 and S2.