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. 2018 Jul 9;145(13):dev160465. doi: 10.1242/dev.160465

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© 2018. Published by The Company of Biologists Ltd

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Fig. 4. — Normal numbers of Sertoli cells and Leydig cells detected within the mutant testes. (A-F) Representative cross-sections of 30 (A), 90 (B) and 180 dpp (C) control RAR-DN-Flox/Flox; Cyp17iCre-negative testes, and 30 (D), 90 (E) and 180 dpp (F) mutant RAR-DN-Flox/Flox; Cyp17iCre-positive testes stained for SOX9 (brown staining, red arrows) by immunohistochemistry. (G-L) The cross-sections of 30 (G), 90 (H) and 180 dpp (I) control RAR-DN-Flox/Flox; Cyp17iCre-negative testes, and 30 (J), 90 (K) and 180 dpp (L) mutant RAR-DN-Flox/Flox; Cyp17iCre-positive testes were also stained for HSD3B1 (brown staining, blue arrows). (M,N) Graphs show the quantification of numbers of Sertoli cells (M) and Leydig cells (N) per testis tubule (y-axis) in the 90 dpp control (black bars) and mutant (gray bars) testes (n=3 for each genotype). Scale bars: 50 μm. Black arrows indicate vacuoles, hashes indicate degraded tubules and asterisks indicate missing elongated spermatids. Data are mean±s.e.m.