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. 2018 Jul 20;18:78. doi: 10.1186/s12866-018-1201-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — a Antibody titers in vaccinated and unvaccinated pullets during rearing. Arrows indicate the timing of vaccine administration. At each sampling point, blood (n = 15 each from vaccinated and unvaccinated groups) from jugular vein was collected. Data was analysed by ANOVA. No significant differences were detected between vaccinated and unvaccinated group at week 4 and 8. At week 13, antibody titers in vaccinated flock was significantly higher (p < 0.01) compared to unvaccinated flock (b) Antibody titers in vaccinated and unvaccinated hens during early lay. At each sampling point, blood (n = 10 each from vaccinated and unvaccinated groups) from jugular vein was collected. Data was analysed by ANOVA. Antibody titers in vaccinated flock were significantly higher (p < 0.0001) compared to unvaccinated flock