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. 2018 Jul 20;15:50. doi: 10.1186/s12977-018-0431-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — CT-deleted 4-2.J41 Env is cleaved. a Western blot analysis of cell lysates (left panel) and neutravidin-agarose precipitates (right panel) of cell surface biotinylated mock transfected, 4-2.J41_WT and 4-2.J41ΔCT₇₁₁ Env transfected 293T cell lysates are shown. b Spontaneous and sCD4-induced gp120 shedding of 4-2.J41_WT and 4-2.J41ΔCT₇₁₁ Env transfected 293T cells incubated separately with 20 ug/ml of sCD4 determined by ELISA. c FACS-based binding of 4-2.J41_WT and 4-2.J41ΔCT₇₁₁ on cell surface to trimer-selective, cleavage-specific PGT151 bNAb. The Env, 4-2.J41_SEKS, a cleavage defective, non-native form of 4-2.J41 Env was used as a negative control. The error bars indicate the standard mean obtained from three independent experiments performed in duplicates, P < 0.0001