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. 2018 Jul 19;19(3):1470320318789332. doi: 10.1177/1470320318789332

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Decreased NO production in aortic rings from ageing females. To evaluate the effect of E2 on NO production, aortic arch longitudinal sections from young, elderly and elderly+E2 were analyzed. (a) Representative microphotographs using DAF-FM (green) in aortic arch from young (n=4), old (n=4) and old E2-treated (seven days, 1 µg/day, n=3) female mice. (b) Graph represents NO production by endothelial cells from aortic rings. Elderly females presented a decreased NO production compared with young females. Statistical analyses: ANOVA followed by Newman–Keuls Multiple Comparison post hoc test was performed as statistical analysis. #p < 0.05 compared to the control group. DAF-FM: 4,5-diaminoﬂuorescein-diacetate probe; L: lumen.