Table 1. Target bacteria, oligonucleotide primers/probes, and annealing temperatures used for quantitative PCR analysis.
| Target | Primer type | Sequence (5′-3′) | Annealing (°C) |
|---|---|---|---|
| Universal bacteria | Forward | CCTACGGGAGGCAGCAGT | 59 |
| Reverse | ATTACCGCGGCTGCTGG | ||
| Blautia spp. | Forward | TCTGATGTGAAAGGCTGGGGCTTA | 56 |
| Reverse | GGCTTAGCCACCCGACACCTA | ||
| Clostridium hiranonis | Forward | AGTAAGCTCCTGATACTGTCT | 50 |
| Reverse | AGGGAAAGAGGAGATTAGTCC | ||
| Escherichia coli | Forward | GTTAATACCTTTGCTCATTGA | 55 |
| Reverse | ACCAGGGTATCTAATCCTGTT | ||
| Faecalibacterium spp. | Forward | GAAGGCGGCCTACTGGGCAC | 60 |
| Reverse | GTGCAGGCGAGTTGCAGCCT | ||
| Fusobacteria spp. | Forward | KGGGCTCAACMCMGTATTGCGT | 51 |
| Reverse | TCGCGTTAGCTTGGGCGCTG | ||
| Streptococcus spp. | Forward | TTATTTGAAAGGGGCAATTGCT | 54 |
| Reverse | GTGAACTTTCCACTCTCACAC | ||
| Turicibacter spp. | Forward | CAGACGGGGACAACGATTGGA | 63 |
| Reverse | TACGCATCGTCGCCTTGGTA |