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. Author manuscript; available in PMC: 2019 Jan 24.
Published in final edited form as: J Am Chem Soc. 2017 Sep 25;140(3):916–918. doi: 10.1021/jacs.7b05161

Table 1.

Substrate Activity of Cholesterol and 3HPC with Wild-Type and D303 Mutant HhCa

substrate
cholesterol 3HPC
wild-type HhC
kmax(s−1) 4.70 × 10−3 1.21 × 10−3
KM(μM) 1.87 1.54
kmax/KM(M−1s−1) 2.53 × 103 785
D303A HhC
kmax(s−1) nd 1.03 × 10−3
Kmax(μM) nd 3.47
kmax/KM(M−1s−1) <0.27 296
a

Standard errors were less than or equal to ±14% for KM values and ±20% for kmax values. n ≥ 3.