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. 2018 Jun 8;69(16):3949–3961. doi: 10.1093/jxb/ery214

Fig. 3.

Fig. 3.

Map-based cloning of the WSL5 allele. (A) The WSL5 locus was mapped to a 180 kb region between markers Y17 and Y47 on chromosome 4L. Black arrows represent 22 putative genes in this region; the candidate gene WSL5 (Os04g0684500) is shown by a red arrow. (B) ATG and TGA represent the start and stop codons, respectively. Black boxes indicate the exons, and the white boxes indicate the 3'- and 5'-UTRs. An SNP in the first exon in WSL5 causes a leucine to proline amino acid substitution. (C) Alignment of amino acid sequences with highest identity to the WSL5 protein. The red arrow indicates an amino acid change. (D) Complementation of wsl5 by transformation.