Table 1.
DNA Sequencing Strategy
| Raw reads |
Trimmed Reads |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Type | Insert Size (bp) | Read Length (bp) | No. Reads (paired) | Total Length (Mb) | No. Reads (paired) | Total Length (Mb) | Total Length (% raw) | Read Length (bp, trimmed) | Coverage |
| Paired-end | 300 | 2×100 | 189,876,842 | 37,975 | 185,721,156 | 36,274 | 95.5 | 97.6 | |
| Paired-end | 300 | 2×100 | 195,394,768 | 39,079 | 191,002,987 | 37,319 | 95.5 | 97.7 | |
| Paired-end | 300 | 2×100 | 178,820,287 | 35,764 | 174,954,230 | 34,224 | 95.6 | 98.9 | |
| Total | 564,091,897 | 112,818 | 551,678,373 | 107,818 | 95.6 | 98.1 | 65× | ||
| Mate pair | 5000 | 2×100 | 97,801,148 | 19,560 | 94,350,168 | 18,717 | 95.7 | 99.3 | 11× |
| Pacific Biosciences Long reads | 4,406.4 (average) | 103,096 | 454 | 0.27× | |||||
| assembled transcriptome | 1,170.9 (average) | 285,260 | 334 | ||||||
| 301–50,048 (min–max) | |||||||||