Fig. 2.
Virus-induced gene silencing (VIGS) of HvCCD7 in barley. (a) HvCCD7 transcript levels in leaf tissue as determined by qRT-PCR. Serine/threonine protein phosphatase PP2A-4 was used as the reference gene. The box-plots represent the results from three independent samples. (b) The tillering phenotypes of BSMV-MCS and BSMV-HvCCD7-silenced plants and the reference variety Black Hulless. The scale bar is 10 cm. (c) Total tiller number per plant. The box-plots represent of the results from 12 plants per genotype. Significant differences according to Student’s t-test are indicated: *P˂0.05, **P˂0.01.