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. 2018 May 18;69(16):4065–4082. doi: 10.1093/jxb/ery190

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Expression of ethylene biosynthesis genes. Relative transcript levels of ACS4 (A and B), ACS7 (C and D), ACO2 (E and F), and ACO4 (G and H) in the root tissue waterlogged for 1, 7, 14, and 28 d (A, C, E, and G) and stem node tissue waterlogged for 7, 14, and 28 d (B, D, F, H), and their respective controls. Transcript levels of the ACS and ACO genes in both tissues were determined using Ta18SrRNA as the reference gene and expressed relative to the transcript levels of ACS4 and ACO2 in control roots at 1 d after the start of waterlogging, respectively, which were arbitrarily set a value of 1. Data are means of three biological replicates ±SE. Asterisks denote a statistically significant difference in transcript levels between tissue samples derived from control and waterlogged plants within each duration of waterlogging (P<0.05; Student t-test). WL, waterlogging. ACS2 and ACO5 exhibited either very minimal or undetectable levels of expression irrespective of the tissue type or growth conditions, as shown in Supplementary Table S3.