Table 1. Efficiency of HAC for single insert cloning.

Cloning of gfp into pUC19
	Stopped with EDTA			Repaired with dNTPs
	Fluorescent colonies	Total colonies	% success	Fluorescent colonies	Total colonies	% success
15 bp overhangs	217	226	96	143	175	82
20 bp overhangs	458	467	98	367	415	88
25 bp overhangs	754	761	99	625	686	91