Table 1.
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
---|---|---|
Alp | CATGTTCCTGGGAGATGGTATG | GTGTTGTACGTCTTGGAGAGAG |
Col1 | TGTTGGTCCTGCTGGCAAGAATG | GTCACCTTGTTCGCCTGTCTCAC |
Msx2 | CCTGCACCCTGAGGAAACAC | TGCCTTAGCCCTTCGGTTCT |
Ocn | ACCCTCTCTCTGCTCACTCTGC | CCTTACTGCCCTCCTGCTTGG |
GAPDH | GGGAAACCCATCACCATCTT | CCAGTAGACTCCACGACATACT |
Abbreviation: RT-PCR, real-time quantitative reverse transcription polymerase chain reaction.