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. Author manuscript; available in PMC: 2018 Sep 14.
Published in final edited form as: Nature. 2018 Mar 14;555(7697):529–533. doi: 10.1038/nature25976

Figure 1. Presence and distribution of passage cells in Arabidopsis.

Figure 1

a) Representative suberised endodermis and xylem pole pericycle visualized by a suberin (GPAT5) and xylem pole pericycle (XPP) reporter using plasma membrane-localised mCitrine-SYP122 or 3mCherry-SYP122 reporters, respectively. Asterisks mark passage cell. b) Scoring of lateral root primordia (white squares) and passage cells (black circles) from start of fully suberised zone (0 %) to hypocotyl junction (100%). 5 % binning of data from top panel (grey bars) and trend of passage cell density along the suberised zone (red line) c) Passage cell occurrence in Col-0 and mutants (ahp6-1 and log4). d) Suberisation in Col-0 and mutants (ahp6-1 and log4). en; Endodermis, nd; not detected, xpp; Xylem pole pericycle. Individual letters highlight significant differences between groups. Bar graphs represent mean ± SD, boxplot centers show median. In all graphs, dots represent individual data points. Notice that for all stacked graphs there are 3 measurements per root (unsuberised zone (white), patchy zone (grey) and suberised zone (yellow)). Individual letters show significantly different groups according to a post hoc Bonferroni-adjusted paired two-sided T-test. For more information on Data plots see the statistics and reproducibility section. For a) the image is representative of 5 independent lines. n represents independent biological samples. For individual P values see supplementary table 2. Scale bars: 25 μm.