Extended Data Figure 5. Manipulation of suberisation through signaling repression.

a) Representative pictures of Fluorol Yellow staining of suberin in lines expressing ARR10_EAR-GFP in all differentiated endodermal cells driven by the ELTP or CASP1 promotors. b) Occurrence of passage cells in lines expressing ARR10_EAR-GFP in all differentiated endodermal cells using the ELTP and CASP1 promotor. c) Behavior of suberin in lines expressing ARR10_EAR-GFP in all differentiated endodermal cells using the ELTP and CASP1 promotor. d) Behavior of suberin in 5-day-old plants expressing ARR10_EAR-GFP in all differentiated endodermal cells using the ELTP and CASP1 promotor. Dots represent individual datapoints. Red dots represent individual datapoints. e) Behavior of suberin in 5-day-old plants expressing ARR10_EAR-GFP in all differentiated endodermal cells using the ELTP and CASP1 promotor when plants were germinated on either cytokinin (BA) or abscicic acid (ABA). Red dots represent individual datapoints. Notice that for all stacked graphs there are 3 measurements per root (unsuberised zone (white), patchy zone (grey) and suberised zone (yellow)). Bar graphs represent mean ± SD, boxplots show median. Individual letters show significantly different groups according to a post hoc Bonferroni-adjusted paired two-sided T-test. For more information on data plots see the statistics and reproducibility section. In b) the image is representative of 9 independent lines. All stainings were repeated 3 times. n represents independent biological samples. Scale bars represent 50 μm.