Figure 7.

Upregulation of Slc7a7 mRNA upon ablation of LAT2 in kidney. AA transport system and protein and gene names are indicated. The expression of particular mRNA variants (1, 2, or 3) or the complete set of variants detected together (C) was analyzed in homozygous male mice for the indicated genotypes. When not listed, variants have not been described for that transporter gene, with the exception of CD98hc mRNA variants that have not been analyzed. Green/red table cells indicate gene up-/downregulation, respectively, in comparison with the control condition (wild-type mice under 20% protein diet). Gray table cells indicate no significant changes in gene expression. Expression results of the ablated transporters, TAT1 and LAT2, are shown separately at the bottom of the table, confirming the loss-of-function of each model. ACTB was used as a reference gene (see the Methods section). Data are from 5–8 male mice per experimental group at 3–4 months of age and after 11 days with the indicated experimental diet. Statistical significance of expression ratios is indicated according to Boostratio,³⁷ as follows: ~ for P≤0.1 (trend); *P≤0.05; **P≤0.01; ***P≤0.001; and ****P<0.001. No expression was detected for: Slc38a1 isoform 3, Slc43a1 isoform 3, Slc6a15 common design for all variants, and Slc7a9 isoform 2. WT denotes wild type and dKO denotes dKO LAT2-TAT1 homozygotes.