Figure 10.

Intercalated cells (ICs) contribute to the increment in BP observed with 2 Nedd4–2 gene ablation. A shows mean arterial pressure (MAP) measured by radiotelemetry after 7 days of a 4% NaCl diet. As shown, MAP was higher in the IC Nedd4–2 null mice (n=4) relative to wild-type littermates (n=5) during both awake (dark period; 6 pm to 6 am) and asleep (light period; 6 am to 6 pm) periods. B shows systolic BP measured by tail cuff in wild-type (Slc26a4^+/+/Nedd4–2^+/+) mice, pendrin null (Slc26a4^−/−/Nedd4–2^+/+) mice, Nedd4–2 null (Slc26a4^+/+/Nedd4–2^−/−) mice, and mice that were both pendrin and Nedd4–2 null (Slc26a4^−/−/Nedd4–2^−/−) after consuming a 4% NaCl diet ad libitum for 6 days. Measurements were made in five mice from each group. *P<0.05.