Table 3.
Effect of IC Nedd4–2 gene ablation on apical plasma membrane and cytoplasm pendrin abundance in mouse cortical collecting duct and connecting tubule
| Type B | Non-A, Non-B | |||
|---|---|---|---|---|
| Wild Type | IC Nedd4–2 Knockout | Wild Type | IC Nedd4–2 Knockout | |
| No. of mice studied | 8 | 9 | 4 | 5 |
| Apical plasma membrane gold label, gold particles per cell | 7.84±1.59 | 13.1±3.0 | 26.5±4.8 | 47.6±4.1a |
| Cytoplasmic gold, gold particles in cytoplasm per cell | 71.1±1.28 | 53.6±0.76 | 76.0±19.8 | 76.6±8.9 |
| Total gold | 79.0±13.6 | 66.7±8.8 | 102±22 | 124±11 |
| Ratio of apical plasma membrane to cytoplasm pendrin label, ×10−1 | 1.24±0.29 | 2.63±0.51a | 4.6±2.0 | 6.4±0.7 |
| Apical plasma membrane boundary length, millimeters ×10−2 | 0.72±0.07 | 1.02±0.11a | 3.04±0.49 | 4.49±0.36a |
| Apical plasma membrane label density, gold particles per 1 mm apical plasma membrane boundary length ×103 | 1.27±0.39 | 1.19±0.18 | 1.02±0.09 | 1.17±0.15 |
| Cell area, millimeters squared ×10−5 | 4.39±0.20 | 4.65±0.33 | 4.86±0.43 | 4.85±0.55 |
| Cytoplasmic label density, gold particles ×106 per 1 mm2 cytoplasmic area | 1.69±0.36 | 1.19±1.71 | 1.59±0.32 | 1.66±0.19 |
Values were determined using immunogold cytochemistry with morphometric analysis and represent the means±SEM. Values were compared with an unpaired, two-tailed t test. Mice consumed the NaCl-rich diet (1.4 mEq/d NaCl) for 7 d before being euthanized. IC, intercalated cell; Nedd4–2, neuronal precursor cell expressed developmentally downregulated 4–2.
a
P<0.05.