Figure 2.
Compared to FH, HDM-FH constructs demonstrated improved or comparable fluid-phase cofactor activity. C3b (700 nM), FI (20 nM), and increasing concentrations of (A) FH, (B) FH1–5^18–20, (C) FHR1–2^1–5^18–20, or (D) FH1–5^18–20^R1–2 were incubated in solution at 37°C for 1 hour. C3b breakdown was analyzed by SDS-PAGE and Coomassie staining. Disappearance of C3α’-110 band and the appearances of C3α’-68, -46, and -43 bands were indicative of the C3b proteolytic inactivation. One experiment, representative of two independent experiments, is shown.