Figure 6.

HDM-FH reduces C3 deposits and remains bound in the glomerulus for greater than 48hrs. Representative images are shown of mouse kidneys stained with a goat anti–mC3b-FITC (MP Biomedical) to detect glomerular C3 staining (green) and OX-24-Dylight 650 (generated in-house), which binds FH SCR5 (an integral epitope in all agents used in this experiment). OX-24 staining at 24 hours (red) or 48 hours (purple) after a single injection ip of the agent. Nuclei were stained with DAPI (blue). Original magnification, ×10; a scale bar is shown. DAPI, 4′,6-diamidino-2-phenylindole; PBS, phosphate-buffered saline.