Capn5 is expressed in differentiated photoreceptors of the larval zebrafish retina. (A–B′) FISH showing expression of capn5a in the zebrafish retina at 5 dpf. Expression was detected in the ONL (arrows). (B–B′) show an enlarged image of the boxed area in (A–A′). (C–D′) FISH for expression of capn5b in the 5 dpf zebrafish retina. Expression was also detected in the ONL (arrows). (D–D′) show an enlarged image of the boxed area in (C–C′). (E–F′) IHC showing expression of Capn5a/b in the OPL and inner segments of the zebrafish photoreceptors at 5 dpf (arrows). (F–F′) show an enlarged image of the boxed region in (E–E′). GCL, ganglion cell layer; L, lens (the red fluorescence in the lens is nonspecific autofluorescence). Scale bars: 50 μm in (A), (C), and (E) and 100 μm in (B), (D), and (F).