Figure 5.

Cornea NV analysis in India ink-stained corneas. The flat-mounted corneas were stained with India ink and photographed under stereomicroscope on day 7 after the alkali burn. (A) Representative photos showed the new vessels stained by India ink. (B) The comparison of NV area/total cornea (%) between the two groups. The areas of corneal NV and the total cornea were measured and calculated with the Olympus CellSens Standard 1.17 software. The mean percentages of area of NV/total cornea were 52.6% ± 18.4% and 76.3% ± 8.6% in the treated and vehicle control groups, respectively. The NV area of rat cornea in the DAID group was significantly decreased compared to that in vehicle group (mean ± SD, n = 7, *P = 0.019).