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. 2018 Jun 5;67(8):1589–1603. doi: 10.2337/db17-1089

Figure 8.

Figure 8

TACI-deficient Mϕs sensitize wild-type (WT) adipocytes to insulin stimulation. A: Mean TNF-α, IL-6, and IL-10 levels in culture supernatants of WT and TACI-deficient VAT Mϕs are shown (n = 15 for each group). B: HFD-fed WT mouse SAT fractions were cultured in CM collected from VAT Mϕs of HFD-fed WT or TACI KO mice for 3 days and then starved for 24 h. After starvation, cells were stimulated with insulin and extracted proteins were subjected to Western blot analysis. One of three experiments with similar results is shown. C: Ratios of P-IRS-1, P-Akt, and P-ERK1/2 band densities in CM-stimulated VAT fractions to their corresponding total proteins were calculated. Mean fold induction relative to basal levels from three experiments is shown. D: WT mouse VAT fractions were cultured for 3 days in CM from HFD-fed TACI-deficient Mϕs with IgG1 or α-IL-10 antibodies. After 3 days of starvation and insulin stimulation, proteins were extracted and subjected to Western blot analysis. One of three experiments with similar results is shown. ns, not significant. *P < 0.05; **P < 0.01; ***P < 0.001.