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. 2018 Jun 22;7:e34843. doi: 10.7554/eLife.34843

Figure 5. Cleavage of the βI domain disulfide impairs fibrinogen binding.

Figure 5.

(A) Expression of wild-type (64.8% of cells) and β3 C177,184S disulfide mutant αIIbβ3 integrin (82.9% of cells) on BHK cells from staining with fluorescene-conjugated anti-CD61 antibody and flow cytometry. Transfection with empty vector served as negative control. (B) Binding of soluble fibrinogen-AF647 (0.1 mg/mL) to BHK cells expressing wild-type or C177,184S mutant αIIbβ3 integrin. Binding is expressed as the % of CD61+ cells that bound fibrinogen. Data points and errors (SD) are from four separate experiments. (C) Adhesion of BHK cells expressing wild-type or C177,184S mutant αIIbβ3 integrin to immobilized fibrinogen. Experiments were performed in triplicate, three fields were analyzed per well and errors are SD. *p<0.05, ***p<0.005, ****p<0.001; assessed by unpaired, two-tailed Student’s t-test.