(
A) GFP::PH showing the plasma membrane during successful cell division in EMS and P2 cells from control and
formincyk-1(ts) mutant embryos. 13 × 2.5 μm Z-sections were rotated 90° (see schematic) to show an X-Z projection of the division plane for each time point. White numbers = Time (sec) after anaphase onset. (
B) Schematic showing measurement of contractile ring diameter and normalization relative to initial (metaphase) cell diameter. (
C) Graphs showing contractile ring constriction over time for EMS and P2 cells in control and
formincyk-1(ts) mutant embryos. All replicates are shown on the left. Cells that complete cytokinesis are shown on the right (individual replicates (thin lines); mean (thick lines)). EMS and P2 cells underwent contractile ring constriction more slowly in
formincyk-1(ts) mutant embryos compared with in control embryos. Time 0 = last time point before anaphase onset; error bars, mean ± SEM; temperature, 16 or 26°C; scale bar = 10 µm. See also
Supplementary file 1.