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. 2018 Jul 13;7:e37963. doi: 10.7554/eLife.37963

Figure 2. Disruption of Slc6a14 in F508del CF mice leads to decrease in weight gain post weaning.

(a) Dorsal view of CF (CftrF508del/F508del) mice and double mutant (CftrF508del/F508del; Slc6a14(-/y)) mice. Both were day 30 male mice. (b) Bar graph represents weights of Wt, CftrF508del/F508del, Slc6a14(-/y) and double mutant (CftrF508del/F508del; Slc6a14(-/y)) mice at day 34 (mean ± SEM). Unpaired t-test was performed (#p=0.005, *p=0.04; n ≥ 4 mice for each genotype). (c) Bar graph represents Body Mass Index (BMI) of mice at day 34 (mean ± SEM). Unpaired t-test was performed (*p=0.03; n > 5 mice for each genotype). (d) The graph represents Kaplan-Meier survival curves for the four genotypes of mice. Double mutant mice were susceptible to death post-weaning. There was no statistical difference in the survival, between Wt and F508del-Cftr mice, However, the survival of double mutant mice was significantly lower than Slc6a14(-/y) mice (Log Rank test, p=0.0036; Wt mice n = 14, Slc6a14(-/y)n = 15, CftrF508del/F508deln = 10, double mutant - CftrF508del/F508del; Slc6a14(-/y)n = 11).

Figure 2.

Figure 2—figure supplement 1. Line graph represents weight gain over time in Wt, CftrF508del/F508del, Slc6a14(-/y) and double mutant (CftrF508del/F508del; Slc6a14(-/y)) mice.

Figure 2—figure supplement 1.

Figure 2—figure supplement 2. Deletion of Slc6a14 in CF mice does not cause a change in serum IGF-1 levels.

Figure 2—figure supplement 2.

(a) Standard curve for ELISA-based detection of IGF-1 levels. Purified IGF-1 was used generate the standard curve. Sigmoidal 4PL curve fit was used as recommended by the manufacturer. Goodness of fit as measured by R2 was 0.9999. (b) ELISA-based IGF-1 detection assay was performed on serum samples obtained from 56 day old mice (i) or 30 day old mice (ii), and actual concentrations interpolated from the standard curve. Bar graphs represent mean ± SD. Serum was collected from Wt, CF (CftrF508del/F508del), Slc6a14(-/y) and double mutant (CftrF508del/F508del; Slc6a14(-/y)) mice on FVB background. One-way ANOVA with Tukey’s multiple comparison test was performed (*p=0.048, #p=0.015, **p=0.0422, n ≥ 6 for each genotype for day 56 mice, n ≥ 3 for day 30 CF and double mutant mice).