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. 2018 Jul 13;7:e37963. doi: 10.7554/eLife.37963

Figure 3. Slc6a14 disruption results in an increase in smooth muscle thickness in the ileum and colon.

(a) Representative distal ileal sections stained with Alcian blue, for Wt, CftrF508del/F508del and double mutant (CftrF508del/F508del; Slc6a14(-/y)) mice. (b) Representative colonic sections stained with Alcian blue, for Wt, CftrF508del/F508del and double mutant (CftrF508del/F508del; Slc6a14(-/y)) mice. (c) Bar graph represents ileal smooth muscle area relative to total section area for each genotype (mean ± SD). One-Way ANOVA with Tukey’s multiple comparison test was performed (**p=0.007, ****p<0.0001, n ≥ 4 mice for each genotype). (d) Bar graph represents colon smooth muscle area relative to total section area for each genotype (mean ± SD). One-Way ANOVA with Tukey’s multiple comparison test was performed (*p=0.042, n ≥ 4 mice for each genotype).

Figure 3.

Figure 3—figure supplement 1. Representative intestinal section with annotations (green line) made in 3DHISTECH software are shown. These annotations were used to calculate relevant areas of tissue layers.

Figure 3—figure supplement 1.