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. Author manuscript; available in PMC: 2018 Oct 26.
Published in final edited form as: Oncogene. 2018 Apr 26;37(29):4013–4032. doi: 10.1038/s41388-018-0243-y

Figure 2. Hepcidin is increased in MCF-7 breast cancer spheroids compared to non-tumor spheroids and is induced from 2D to 3D culture of breast cancer cells.

Figure 2

(A) Phase-contrast imaging of MCF-7 and MCF-10A cells grown in 2D and 3D and fluorescent imaging of spheroids stained with 2μM calcein-AM. (B–D) RT-qPCR of hepcidin mRNA (normalized to cyclophilin A) in MCF-7 and MCF-10A cells grown in 2D and 3D. (E–G) Western blot of pro-hepcidin using Cyclophilin B as internal control. Scale bar: 10μm (2D), 200μm (3D)