. Author manuscript; available in PMC: 2019 Jul 20.

Published in final edited form as: ACS Chem Biol. 2018 Mar 5;13(7):1872–1879. doi: 10.1021/acschembio.7b01019

Table 1.

Characteristics of Selected Sensor Variants

sequence	K_D^a (nM)	Δsignal^b	Hill coefficient^a	mCh/Venus^c
EcYcgR^d	354 ± 18	3	2.3 ± 0.23	1
Selected for High Affinity
TmYcgR	<50	6.1	1.5 ± 0.04	1.7
TuYcgR	<50	6.3	1.4 ± 0.04	2.7
TbYcgR	<50	7.9	1.6 ± 0.1	1.4
CpYcgR	<50	6.3	1.9 ± 0.1	1.7
CbYcgR	<50	18.8	1.2 ± 0.04	0.73
TcYcgR	59 ± 2	4.6	1.7 ± 0.1	1.6
BvYcgR	105 ± 2	4.9	1.9 ± 0.05	2.7
Selected for High Signal Change
DnYcgR	303 ± 15	39.3	1.8 ± 0.1	0.61
ToYcgR	379 ± 23	24.5	2.2 ± 0.2	1.1
Selected for Negative Signal Change
PtYcgR	<50	−7.7	−1.8 ± 0.1	1.6
BtYcgR	286 ± 17	−89	−2 ± 0.2	0.52