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. 2018 Jul 20;8:10983. doi: 10.1038/s41598-018-29278-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reporter gene analysis of EgrNAC26 and EgrNAC61 promoters. (a) Promoter analysis in Arabidopsis seedlings. Top panel, GUS histochemical staining in seedling roots. Insets show the positions of protoxylem (white chevrons) and metaxylem (yellow chevrons) vessels. 35 Spro, CaMV 35S promoter (positive control). Bottom panel, corresponding GFP confocal microscopy analysis of seedling shoot tips for each construct. Cot, cotyledon; Hy, hypocotyl. (b) EgrNAC61 promoter::GUS analysis of internodes 1 and 5 in transgenic poplar plants. Bottom panel, phloroglucinol-stained GUS sections. CC, cork cambium; Co, cortex; PF, phloem fibres; Ph, phellem; SX, secondary xylem; VC, vascular cambium.