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. 2018 Jul 20;8:11013. doi: 10.1038/s41598-018-29044-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of Uncaria tomentosa (UT) on metabolic parameters of ob/ob animals. Adult ob/ob mice fed with standard chow were divided into two groups: control (ob/ob) and UT (ob/ob+UT), which received 50 mg.kg⁻¹ b.w. of crude plant extract (UT) diluted with saline, for 5 consecutive days, once a day, via gavage. The animals were isolated in CLAMS chambers. After two days of adaptation, the mice received either vehicle (saline) or UT, 50 mg.kg⁻¹ b.w., once a day for 5 days (ob/ob+UT), via gavage. During the last 3 days, food intake to calculate the caloric intake (A), O₂ consumption (VO₂) (C), CO₂ production (VCO₂) (D), and the respiratory coefficient (RER) (E) were measured. Body mass index was calculated at the end of the period (B). The results are represented as mean ± SEM, n = 4 to 5. The statistical difference was analyzed by Student’s t-test or two-way ANOVA.