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. 2018 Jul 20;8:11013. doi: 10.1038/s41598-018-29044-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of Uncaria tomentosa (UT) on the inflammatory signaling pathway in the liver of ob/ob mice. Samples of livers from ob/ob mice that received either vehicle (ob/ob) or UT extract (ob/ob+UT) were used for Western blot analysis (A–D) or for mRNA expression (E–H). Phosphorylated proteins were calculated by the ratio of the optical densitometry of phosphorylated and total protein expression. Protein expression data for other proteins was normalized to β-actin, n = 5. Photomicrographs of monocyte/macrophage marker F4/80 immunohistochemistry (brown color) in the liver of ob/ob mice under treatment with UT, n = 4 (20× all photomicrographs, Scale bar = 50 μm) (I,J). Data are presented as mean ± SEM, respectively. The statistical difference as indicated by Student’s t-test was, *p < 0.05.