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. 2018 Jul 20;8:10978. doi: 10.1038/s41598-018-29379-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Correlated findings of loose clusters of micro calcifications in the intima in location-matched pairs of tomographic and histological images. In x-ray tomographic images calcifications appear as brighter dots and patches due to their higher mass density; adipose tissue such as foam cells appear as dark patches due to their lower density. The histological slides were stained with von Kossa stain for calcifications which appear as dark brown or black color. (A) and (B) Scattered clusters of micro calcifications (solid arrows) of an atherosclerotic lesion are seen in matching locations of the paired images, together with foam cells in the neo intima (open arrows). (C) and (D) A second lesion shows scattered intimal micro calcifications (solid arrows) in matching locations between the x-ray and histological images. Scalebars are 250 µm.